Microvascular vessel anatomosis
Considerations
Learning
Technique
Preparation
Flush the artery and venous system with hep saline prior to (open system)
Inset first (Dr. Gurushanthaiah/Fong)
Anastomosis first (Dr. Moon)
Prep vessels
trim the adventitia from the edge of planned anastomosis
triangulate the vessel by holding only the adventitia.
check arterial and venous flow
Procedure
Artery before vein
Arterial
9-0 nylon non traumatic needle (non cutting)
Vessel clamps on both sides
Make sure the edges are smooth and not ragged
Make sure end is not near a point of arborization
Place vessels in the arena (not sure what it's called) with background
Place you 6 and 12 oclock stitches and leave the tail long so you can wrap the tail around the anchors of the arena
Place you next two stitches evenly at 7 and 11 oclock. Tie each one as you throw them.
Next, the 8, 9, 10 oclock stitches you throw but do not tie down to allow for you to look into the vessel lumens as you place the stitches.
once all three are placed, then you can tie them down.
Flip the vessels over 180 and repeat.
As a resident, most likely you will not be doing the artery. You can assist by keeping things dry with Weck-cel sponges, irrigate with hep saline when needed, cut with straight microscissors
Venous
Use of coupler
largest coupler that will fit the smaller of the vessels.
size the vessel with the sizer to choose the coupler
Place first of two vessels through the ring of the coupler
Use the angled micropickups with the gap in the middle (one in each hand) to place the edge of the vein and impale the edge over the coupler pins
with the pickups closed, then place the gap within the pickups on the coupler pin and gently push down.
push down very gently to avoid macerating the intima
want to triangulate the edges first then get then impale the rest of the edges of the vessel.
Make sure the edges of the vein are everted
Repeat for the other vessel
Once the veins are properly seated, turn the knob on the coupler applicator all the way until the coupler/vessels comes out of the applicator
Give the coupler a little extra squeeze with hemostat
Vessel clamps off
Irrigate the vessels with 2% lidocaine
Sample operative report
Post-op
Pearls & Pitfalls
High-Yield
Links
Iowa Protocols - Microsurgical technique - Includes videos
UTMB Grand Rounds - Microvascular Free Tissue Transfer
Microsurgical Technique for 1mm Vessel End to End Anastomosis - Article plus 40 minute video
YouTube (7:31) - Microsurgical arterial end-to-end anastomosis; basic technique by AboutMicrosurgery
YouTube (18:37) - MICROSURGERY - End To End Arterial Arterial Anastomosis by World Microsurgery
YouTube (20:53) - The Mechanics of Microsurgical Suturing by Columbia Orthopedics (excellent instruction)